Lab Record Biology you And Lab Essay

EnzymesВ В

IntroductionВ

ThisВ studyВ allowsВ theВ investigationВ ofВ enzymaticВ reactionsВ behavior. В В AnВ enzymeВ isВ aВ proteinВ catalystВ reactionВ byВ loweringВ theВ activationВ energyВ requiredВ forВ thatВ reaction. В TheВ enzymeВ isВ unalteredВ atВ theВ completionВ ofВ theВ reaction. В InВ thisВ stimulationВ theВ amountВ ofВ productВ producedВ duringВ theВ courseВ ofВ anВ enzymaticВ productВ producedВ duringВ theВ courseВ ofВ anВ enzymaticВ reactionВ willВ beВ measured. В В HypothesisВ 1: В

WhatВ isВ theВ estimateВ optimalВ ofВ ph? В

HypothesisВ 2: В

WhatВ isВ theВ estimateВ optimalВ ofВ temperature? В

HypothesisВ 3: В

HowВ doesВ theВ concentrationВ ofВ enzymeВ influenceВ theВ rateВ ofВ reaction? В WhyВ doВ youВ thinkВ В thisВ occurs? В

MaterialsВ В

в—Џ

в—Џ

в—Џ

в—Џ

в—Џ

в—Џ

в—Џ

SubstrateВ solutionВ

CuvetteВ

SpectrophotometerВ

TimerВ

EnzymeВ solutionsВ

PipetteВ forВ transferringВ fluidsВ

ThermometerВ

MethodsВ

в—Џ ThereВ isВ aВ choiceВ ofВ sixВ differentВ enzymeВ & В substrateВ combinationsВ inВ whichВ theВ temperatureВ willВ varyВ atВ whichВ theВ reactionВ asВ wellВ asВ theВ enzymeВ & В substrateВ concentrations. В

pH: В

1 . AВ SUbstrateВ B/В EnzymeВ BВ wasВ selectedВ alongВ withВ settingВ theВ wavelengthВ toВ substrateВ vial/В 680В & В theВ spectrophotometerВ toВ readВ lightВ absorption. В В

2 . TheВ temperatureВ wasВ setВ toВ 25В degreesВ & В setВ toВ differentВ experimentalВ pHВ valuesВ startingВ atВ valueВ 2В throughВ valueВ 10В (allВ evenВ #'s)В

three or more. TransferredВ 25В mlВ ofВ theВ substrateВ BВ intoВ theВ cuvetteВ usingВ theВ pipette. В

5. ReferringВ toВ theВ timerВ toВ determineВ whenВ 1В minuteВ hasВ passedВ transferredВ 5В mlВ ofВ enzymeВ BВ intoВ theВ cuvetteВ andВ quicklyВ intoВ theВ sampleВ holderВ ofВ theВ spectrophotometer. В

5. RecordedВ theВ absorptionВ valueВ atВ theВ 1В minuteВ markВ 6. StartedВ aВ newВ experimentВ byВ removingВ theВ cuvetteВ fromВ theВ spectrophotometerВ andВ clickingВ theВ " NewВ Experiment” buttonВ toВ repeatВ andВ continueВ theВ stepsВ forВ theВ remainingВ pHВ values. В В 7. DeterminedВ theВ estimateВ ofВ optimalВ pHВ afterВ theВ entireВ experimentВ forВ asВ aВ result. В В

Temperature: В

1 . UsedВ theВ sameВ SUbstrate/В EnzymeВ BВ & В setВ theВ pHВ valueВ toВ theВ optimalВ pHВ valueВ -В 4В fromВ theВ pHВ results. В

installment payments on your В TheВ temperatureВ wasВ setВ toВ 10В degreesВ & В setВ toВ differentВ experimentalВ temperaturesВ startingВ atВ valueВ 10В throughВ valueВ 80В (allВ inВ theВ 10'sВ place)В

3. RepeatedВ theВ sameВ stepsВ asВ 4В -В 7В asВ theВ pHВ experimentВ В

ReactionВ Rates: В

1 . UsedВ theВ sameВ SUbstrate/В EnzymeВ B, В setВ theВ pHВ valueВ toВ theВ optimalВ pHВ valueВ -В 4В fromВ theВ pHВ results, В & В setВ theВ temperatureВ toВ theВ optimalВ temperatureВ -В 20В fromВ theВ temperatureВ results. В

2 . TransferredВ 5В mlВ ofВ theВ enzymeВ BВ intoВ theВ cuvetteВ usingВ theВ pipette. В

3. CompletedВ theВ sameВ stepsВ 4В -В 7В asВ theВ pHВ experiment, В butВ recordedВ myВ dataВ basedВ offВ ofВ 10В secondВ intervalsВ throughВ aВ totalВ ofВ 2В minutesВ toВ determineВ theВ absorptionВ value. В В 4. AfterВ completingВ theВ stepsВ transferringВ 5В mlВ ofВ theВ enzymeВ BВ intoВ theВ cuvetteВ usingВ theВ pipette, В repeatedВ theВ stepsВ transferringВ 15В mlВ instead. В В

В 

ResultsВ

pHВ В

Substrate/EnzymeВ BВ

ExperimentalВ pHВ

StartВ TimeВ EndВ TimeВ

AbsorptionВ @В 1В MinuteВ

10: 52: 37В

11: 53: 37В

14: 54: 27В

11: 55: 27В

. 144В

10: 56: 03В

11: 57: 03В

. 137В

doze: 00: 36В

12: 01: 36В

. 067В

10В

doze: 02: 28В

12: goal: 28В

BasedВ onВ theВ results, В theВ estimateВ ofВ optimalВ phВ isВ 4В

В 

TemperatureВ

Substrate/EnzymeВ BВ

ExperimentalВ

Temp. В В

StartВ TimeВ

EndВ TimeВ

AbsorbanceВ @В 1В

minuteВ

10В

12: 07: 59В

12: 08: 59В

. 147В

20В

doze: 10: 01В

12: 10: 01В

. 240В

30В

12: 14: 00В

12: 12-15: 00В

. 032В

40В

12: 15: 41В

12: sixteen: 41В

. 066В

50В

doze: 17: 25В

12: 18: 25В

. 035В

60В

doze: 19: 46В

12: twenty: 46В

70В

12: 21: 26В

12: 22: 26В

80В

12: 24: 00В

12: twenty-five: 00В

BasedВ onВ theВ results, В theВ optimalВ temperatureВ isВ 20В

ReactionВ RatesВ

Substrate/EnzymeВ BВ

TimeВ

5В mlВ EnzymeВ

15В mlВ EnzymeВ

: 10В

. 110В

. 035В

: 20В

. 110В

. 035В

: 30В

. 110В

. 035В

: 40В

. 110В...



The Holocaust was the Reaction to Hitler’s Personal Desire for Genocide: Assessment with the Statement Essay

Related

Category

News